Review



exons 12 18  (Addgene inc)


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    Structured Review

    Addgene inc exons 12 18
    Exons 12 18, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/exons+12+18/JJ802%3A+pMAGIC+(L1-R5)+hU6%3A%3ASaCas9+gRNA+scaffold+(Plasmid+%23121812)/pmc06891089-184-63-34
    Average 90 stars, based on 1 article reviews
    exons 12 18 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: CDK1-mediated CENP-C phosphorylation modulates CENP-A binding and mitotic kinetochore localization
    Article Snippet: .. Three single guide RNAs (sgRNAs 1, 2, and 3) against genomic sequence around the start of gCENP-C exon12 were designed (Optimized CRISPR Design; ) and cloned into pX335 plasmid, which has SpCas9 nickase (D10A; Addgene, 54233 [ ]; pX335-gCENP-C 1, 2, and 3). sgRNA 3 was also cloned into pX330 plasmid (Addgene, 42330; pX330-gCENP-C 3). gCENP-C cDNA corresponding to aa 647–864 coded on exons 12–18 fused with GFP and puro R of HisD expressed by β-actin promoter were inserted between ∼1 kb 5′ and 3′ homology arms flanking the start of gCENP-C exon12 and cloned into pBlueScriptII KS (pBSKS-gCENP-C-GFP WT -puro R or HisD). ..

    CRISPR:

    Article Title: CDK1-mediated CENP-C phosphorylation modulates CENP-A binding and mitotic kinetochore localization
    Article Snippet: .. Three single guide RNAs (sgRNAs 1, 2, and 3) against genomic sequence around the start of gCENP-C exon12 were designed (Optimized CRISPR Design; ) and cloned into pX335 plasmid, which has SpCas9 nickase (D10A; Addgene, 54233 [ ]; pX335-gCENP-C 1, 2, and 3). sgRNA 3 was also cloned into pX330 plasmid (Addgene, 42330; pX330-gCENP-C 3). gCENP-C cDNA corresponding to aa 647–864 coded on exons 12–18 fused with GFP and puro R of HisD expressed by β-actin promoter were inserted between ∼1 kb 5′ and 3′ homology arms flanking the start of gCENP-C exon12 and cloned into pBlueScriptII KS (pBSKS-gCENP-C-GFP WT -puro R or HisD). ..

    Clone Assay:

    Article Title: CDK1-mediated CENP-C phosphorylation modulates CENP-A binding and mitotic kinetochore localization
    Article Snippet: .. Three single guide RNAs (sgRNAs 1, 2, and 3) against genomic sequence around the start of gCENP-C exon12 were designed (Optimized CRISPR Design; ) and cloned into pX335 plasmid, which has SpCas9 nickase (D10A; Addgene, 54233 [ ]; pX335-gCENP-C 1, 2, and 3). sgRNA 3 was also cloned into pX330 plasmid (Addgene, 42330; pX330-gCENP-C 3). gCENP-C cDNA corresponding to aa 647–864 coded on exons 12–18 fused with GFP and puro R of HisD expressed by β-actin promoter were inserted between ∼1 kb 5′ and 3′ homology arms flanking the start of gCENP-C exon12 and cloned into pBlueScriptII KS (pBSKS-gCENP-C-GFP WT -puro R or HisD). ..

    Plasmid Preparation:

    Article Title: CDK1-mediated CENP-C phosphorylation modulates CENP-A binding and mitotic kinetochore localization
    Article Snippet: .. Three single guide RNAs (sgRNAs 1, 2, and 3) against genomic sequence around the start of gCENP-C exon12 were designed (Optimized CRISPR Design; ) and cloned into pX335 plasmid, which has SpCas9 nickase (D10A; Addgene, 54233 [ ]; pX335-gCENP-C 1, 2, and 3). sgRNA 3 was also cloned into pX330 plasmid (Addgene, 42330; pX330-gCENP-C 3). gCENP-C cDNA corresponding to aa 647–864 coded on exons 12–18 fused with GFP and puro R of HisD expressed by β-actin promoter were inserted between ∼1 kb 5′ and 3′ homology arms flanking the start of gCENP-C exon12 and cloned into pBlueScriptII KS (pBSKS-gCENP-C-GFP WT -puro R or HisD). ..



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